CGR-list: large plasmid cloning

[Merilyn Hibma]

Hi Everyone,

We are trying to clone a plasmid which is around 15kbp.  I was  
wondering if anyone might have E. coli stains suitable for large  
plasmid cloning, such as the NEB 10-beta strain, which is a derivative  
of DH10B.  I don't know much about this, but perhaps someone else has  
been doing this type of work, or dealing with BACs or cosmids - and  
may be able to help us.

Any suggestions much appreciated,
Merilyn.

Merilyn Hibma
Senior Research Fellow
Dept. Microbiology and Immunology
University of Otago
P O Box 56
(720 Cumberland St.)
Dunedin 9054
New Zealand
Ph. +64 3 479 7726
Fax. +64 3 479 7744