CGR-list: Flp-In T-Rex expression system

[Andrew Mercer]

Does anyone have components of, or experience with, the Invitrogen 
tet inducible expression system, Flp-In T-Rex?

Invitrogen produce a set of premade cell lines carrying  the FRT 
integration site and expressing the tet repressor. The lines are 
called Flp-In T-Rex and have ben established in 293, HeLa, CHO, and 
Jurkat cells.  Does anyone have one of these?

The other components of the system are the inducible expression 
vector, pcDNA5/FRT/TO and the source of the Flp recombinase, pOG44.

It would be great to hear from someone with any of these  (happy to 
share costs) or with experience of this system.

Thanks,
Andy

-- 
Professor Andrew Mercer
Director,Virus Research Unit
Department of Microbiology and Immunology
University of Otago
PO Box 56
Dunedin  9016
New Zealand
Courier Address:  720 Cumberland Street
Phone: (64) (3) 4797730
Mobile: 021 2797730
FAX: (64) (3) 4797744
EMAIL:  andy.mercer at otago.ac.nz